Multidrug Resistant Bacteria in Air Samples from Indoor Environment in Kathmandu, Nepal: A Cross-Sectional Study
DOI:
https://doi.org/10.70280/njph(2025)v2i1.27Keywords:
bacterial contamination, indoor air pollution, antimicrobial resistanceAbstract
Background: Indoor air flora has been linked to several allergic and infectious diseases; hence, monitoring of air quality is key to achieving better health outcomes. It is therefore imperative to monitor air quality for antibiotic-resistant bacteria that are on the rise and are associated with poor human health.
Methods: The study was conducted from June 2019 to November 2020 across 24 indoor sites in Kathmandu Valley, Nepal. Air samples were collected using an impactor air sampler and cultured on various media. Bacterial isolates were identified, and their antibiotic susceptibility patterns were determined using the Kirby-Bauer method per CLSI 2018 guidelines.
Results: A total of 73 culture plates representing 24 samples, were examined. The predominant bacteria included Staphylococcus spp. and Citrobacter freundii. Among 50 isolates, 72% were multidrug- resistant. Gentamycin and Ofloxacin were the most effective antibiotics. Hospitals exhibited the highest microbial load (2729 CFU/m³).
Conclusion: The presence of multidrug-resistant bacteria in indoor environments of Kathmandu highlights a significant public health concern. Continuous monitoring is essential for preventive action.
References
1. Muhammad, M. H., Uba, F., Madinat, R., Muhd, M., Sulaiman,. et al. Isolation and identification of airborne bacteria from Federal University Dutse lecture rooms. International Journal of Science and Technology Research. 2017; 6(09): 16–19.
2. Qing, K., Zhou, X., & Luo, Z, et al. The effects of ventilation, humidity, and temperature on bacterial growth and bacterial genera distribution. International Journal of Environmental Research and Public Health. 2022; 19(22): 15345.
3. Messi, P, Sabia, C, Anacarso, I, Condò, C, Iseppi, R, & Stefani, S, et al. Prevalence of multi-drug-resistant (MDR) bacteria in air samples from indoor and outdoor environments. Aerobiologia. 2015; 31(3): 381–387.
4. Margiorakos, A, Srinivasan, A, Carey, RB, Carmeli, Y, Falagas, ME, Giske, CG, et al. Multidrug- resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012; 18(3):268-81.
5. Schmieder, R., & Edwards, R, et al. Insights into antibiotic resistance through metagenomic approaches. Frontiers in Microbiology. 2012; 7(1):73–89.
6. Bruce N, Perez P, Albalak R. Indoor air pollution in developing countries: a major environmental and public health challenge. Bulletin of the World Health Organization. 2000; 78(9):1078-92.
7. Forbes BA, Sahm DF, Weissfeld AS et al. Bailey & Scott’s Diagnostic Microorganism. 13th ed.St. Louis: Mosby Elsevier; 2014. Available from: https://www.elsevier.com/books/bailey-and-scotts-diagnostic-microbiology/forbes/978-0-323-29942-5
8. Lwanga, S. K., & Lemeshow, S. (1991). Sample size determination in health studies: A practical manual. World Health Organization. Available from: https://iris.who.int/handle/10665/40062AbeBooks+5
9. Centers for Disease Control and Prevention (CDC). (2021). Antibiotic use: Antibiotics for common infections. Available from: https://www.cdc.gov/antibiotic-use/index.html
10. Kunwar, A., Tamrakar, S., Poudel, S., Sharma, S., & Parajuli, P., et al. Bacteriological assessment of the indoor air of different hospitals of Kathmandu District. International Journal of Microbiology. 2019; 17(43):170-5.
11. Abate R, Yalew A., et al. Assessment of bacterial indoor air quality and identification of pathogenic bacteria at Dilla University Hospital, Ethiopia. Environmental Health Insights. 2022;16(1):1-8.
12. Kengne Gounmadje, L., Nyegue, M. A., Djuidje Chatue, I. A., Gonsu Kamga, H., & Etoa, F.-X., et al. Assessment of indoor microbial quality of library’s premise: Case of Central Library of the University Of Yaoundé I. Open Journal Of Preventive Medicine. 2018;8(4):109–120.
13. Gnanamani, A., Hariharan, P., & Paul-Satyaseela, M., et al. Staphylococcus aureus: Overview of bacteriology, clinical diseases, epidemiology, antibiotic resistance and therapeutic approach. Frontiers in Staphylococcus aureus. 2017.
14. Handorean, A., Robertson, C. E., Harris, J. K., Frank, D., Hull, N., Kotter, C., et al. Microbial aerosol liberation from soiled textiles isolated during routine residuals handling in a modern health care setting. Microbiome. 2015; (3): 72.
15. Brągoszewska, E., Biedroń, I., Kozielska, B., & Pastuszka, J. S., et al. Microbiological indoor air quality in an office building in Gliwice, Poland: Analysis of the case study. Air Quality, Atmosphere & Health. 2018; 11(6):729–740.
16. Erlandson, G., Magzamen, S., Carter, E., Sharp, J. L., Reynolds, S. J., & Schaeffer, J. W., et al. Characterization of indoor air quality on a college campus: A pilot study. International Journal of Environmental Research and Public Health. 2019; 16 (15): 17–26.
17. Cartieaux E, Rzepka MA, Cuny D, et al. Qualité de l’air à l’intérieur des écoles. Arch Pédiatr. 2011;18(7):789–96.
18. Canha N, Almeida SM, Freitas MC, Wolterbeek HT, et al. Assessment of bioaerosols in urban and rural primary schools using passive and active sampling methodologies. Arch Environ Prot. 2015;41(4):11–22.
19. Basak S, Singh P, Rajurkar M, et al. Multidrug resistance mechanism in bacteria. J Glob Antimicrob Resist. 2016;4:29–36.
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